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Then RNA-seq reads were quantified using whippet-quant allowing identification of constitutive annotated junctions (CON_ANNO), annotated alternative junctions (ALT_ANNO) and unannotated alternative junctions (ALT_UNIQ) in WT and mutant cells
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Fractional precipitation may be incorporated to remove gross impurities from small sample volumes, and have occasionally been successfully employed in small-scale commercial production
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