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Although numerous topical and systemic therapeutic agents and also non-pharmacologic procedural treatments have been considered in melasma management, however, the commonly available therapeutic options have several limitations including the lack of sufficient clinical effectiveness, risk of relapse, and high rate of unwanted adverse drug reactions

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Phosphate buffered saline (without Ca2+ and Mg2+) containing 5% fetal calf serum (FCS) 5 mM EDTA and 5 μg/ml DNase I