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HIF-1α expression by immunofluorescence 4T1 cells were added in ultra-low adhesion 24-well plates (500 μL, 1 × 10 4 cells for each well) under hypoxic conditions and allowed for the tumorsphere formation

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4.3 Multi-pathway coordinated regulation of ferroptosis Multi-target strategies for ferroptosis protect nerves by strengthening endogenous defenses, mending antioxidant systems, and halting oxidative damage