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Immunohistochemical (IHC) staining For IHC analysis of Nrf2 protein, mouse skin was collected and paraformaldehyde fixed, paraffin-embedded sections of skin tissues (4 μm thick) were mounted on slides coated with 2-aminopropyltriethoxysilane, which then were baked, deparaffinized, rinsed with 3% hydrogen peroxide, and incubated with proteinase K (0.5 mg/mL)