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To validate the specificity of these cell death markers, we evaluated positive and negative controls in tissues and cell lines
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After transfer, membranes were blocked in 1% bovine serum albumin in PBS with 0.1% Tween-20 and probed with anti-PARP (NB100-56599, Novus Biologicals, Littleton, CO), anti-Glutathione (AB19534, Abcam, Cambridge, MA), anti-phospho-IκB-α (9246), anti-IκB-α (9242), anti-phospho-IKK-β (2594), anti-IKK-β (2370), or anti-p65 (8242) (Cell Signaling Technology, Danvers, MA) antibodies
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