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FIGURE 5 Interestingly, one of the unique features of lipid nano formulations amenable to inefficient mRNA loading is a balanced ratio of ionizable lipids, and helper lipids such as 1,2-Dioleoyl-sn-glycero-3-PE (DOPE) and distearoylphosphatidylcholine (DSPC), which promote endosomal membrane fusion, polyethylene glycol (PEG) for improved bioavailability by reducing plasma protein binding, promoting cell-specific interactions toward non-hepatic organs, especially lymph nodes [98]
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