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The aggregates were collected as described above and resuspended at 3.5 ml/million cells in Knockout DMEM (Life Technologies), 20% (vol/vol) Knockout Serum Replacement (Life Technologies), 1% dimethyl sulfoxide (Sigma-Aldrich), nonessential amino acids (NEAA—Life Technologies), 2-mercaptoethanol (Life Technologies) and Glutamax (Life Technologies) and incubated for 5 days, with a medium change every 48 hours
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Volume:5mg – 10 vial lab pack (Save 10%)
The hypothesis was that oxygen released from CPO microspheres would help maintain cell viability in the hypoxic microenvironment, while the release of IL-10 could promote the polarization of macrophages to the M2 phenotype, facilitating the regeneration process
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