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Furthermore, inhibition of GSH synthesis by administration of L-buthionine-(S,R) sulfoximine directly into the mouse hippocampus induced an anxiety-like behavior [67]
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Total RNA was extracted from the cells of each treatment group by the TRIzol method, and the cDNA was synthesized by reverse transcription using total RNA as a template with Reverse Transcription Kit (Takara Bio Inc, Japan) at 37ยฐC for 15 min, 85 ยฐC for 5 s, and 4 ยฐC for 10 min
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