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doi: 10.1210/me.2010-0142 97 ItoSKinoshitaSShiraishiNNakagawaSSekineSFujimoriTet al
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Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) Nedd4-2 (10 µM), 14-3-3η (20 µM), their 1:2 complex, with and without 1 mM Ca 2+ , and 10 µM Nedd4-2 with 1 mM CaCl 2 and LUVs (200 nmol LUVs per 500 pmol Nedd4-2) were preincubated for 30 min at 4 °C (45 min for samples with LUVs) and subjected to H/D exchange
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