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Proteomic analysis of iPSC-derived organoids Organoids were disrupted in 50 µL of lysis buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 20 mM β-glycerol phosphate, 1 mM sodium orthovanadate, 5 mM sodium floride) and sonicated for (20–30 s)
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