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All bladder cancer cell lines were cultured in RPMI-1640 medium (11875093, Thermo Fisher, USA), supplemented with 10% fetal bovine serum (FBS) (10099-141, Gibco, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin (15140122, Thermo Fisher, USA)
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Spectrophotometry assesses the intensity of light after going through a sample, to measure the light absorption or the quantity of chemicals in a solution (Spectrophotometry, 2013, Spectrophotometry, 2020)
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