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Western blotting was performed as previously described 45 , using the following antibodies: rabbit anti-FLAG (1:5,000, F7425, Sigma), rabbit anti-GFP (1:1,000, Invitrogen), mouse anti-Tubulin (1:2,000, T9026, Sigma), mouse anti-CYE-1 (1:2,000, 17C8, a kind gift of E
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