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However, due to the large size of the MBP tag, which brings a heavy metabolic load to the host cell, target proteins tend to remain insoluble or form aggregation-prone regions when MBP tags are removed
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Instead, Cys497′ hTrxR1 is sandwiched between Ser415′ hTrxR1 and Ser111 hTrxR1 , the latter being within hydrogen bond distance (3.6 Å) to a strictly conserved His108 hTrxR1 (Fig
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