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The samples were incubated on ice and centrifuged to separate the serum, which was then collected, transferred to clean tubes, and stored until further experimentation took place [2]

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D: Western blot for GSTP1 after immunoprecipitation with an anti-HPV-16 E7 antibody in control and HPV-16 E7-expressing HaCaT cells shows co-precipitation of GSTP1 only in HPV-16 E7-expressing cells