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Nevertheless, several of these aspects have been successfully sorted out over recent years through the new technologies available in peptides design field: new bioinformatics tools in combination with other approaches such as virtual screening, structure-based drug design, high throughput screening (HTS) and chemical strategies, provide a comprehensive pharmacological description of putative peptides, improving their chemical and physical features (119, 121, 148)
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The ß-klotho in these additional regions likely play important roles in FGF21 pharmacology because ß-klotho knockout in glutamatergic neurons prevents body weight loss due to FGF21 administration [105]
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