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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 °C for 10 seconds and 60 °C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain

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Proc Natl Acad Sci USA 102 , 5535–5540, (2005)