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In a 1988 study by Maquart, Pickart, and colleagues, GHK-Cu stimulated collagen synthesis in cultured fibroblasts at concentrations as low as 10⁻¹² M, with peak effect at 10⁻⁹ M, independent of changes in cell number
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The remaining concentrations of IL-6 (0-100 μg/mL, 2 h) were detected by an enzyme-linked immunosorbent assay (ELISA), after coculturing with MnO2 nanoparticles, MnO2@MNP nanoparticles, and MnO2@TMNP nanoparticles at different concentrations
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