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Fluorescence was measured for 90 min at excitation and emission wavelengths of 485 and 520 nm, respectively, using the Synergy HT multi-detection microplate reader (Biotek Instruments Inc., Winooski, VT, USA)
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No cysteine, so no disulfide bridge The sequence contains no cysteine, so there is no disulfide bond to form, scramble or reduce
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