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For immunohistochemistry, the sections were deparaffinized and rehydrated, processed with microwave antigen retrieval, and incubated overnight with primary antibodies against Ki67 (ab15580), and CD8 alpha (ab217344) (Abcam, Cambridge, UK) at 4 °C
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In addition, triple-negative breast cancer cells increase their CTP and TTP pools in response to doxorubicin or cisplatin in vitro, thereby optimizing DNA repair [83]
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