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doi: 10.1186/s12967-024-05737-z
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Packaging:125 mL Amber Bottle
Following protease treatment, the histones were dialyzed against buffer D (20 mM sodium acetate pH 5.2, 200 mM NaCl, 5 mM β-mercaptoethanol, 1 mM EDTA, and 6 M urea), and the untagged histone was further purified by Resource S cation exchange column chromatography (Cytiva) using buffer D containing a linear gradient of NaCl from 200 to 900 mM
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