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There are also new methods for detecting SDC2 , such as linear target enrichment quantitative methylation-specific real-time PCR (LTE-qMSP), which is highly specific for early detection of SDC2 methylation in fecal DNA in CRC patients ( SDC2 gene methylation detection could be reduced to 0.1% methylation level and 5 ng methylated DNA input (Yue et al., 2022)
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