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Competition gel shift assays The binding affinity of MBP-RR-TZF for ACA11 F4 and deletion derivatives (F1, F4Δ84, F4Δ134) was measured in a gel-shift assay by monitoring dissociation of MBP-RR-TZF from 6-FAM-ARE 19 , which was previously established as a minimal RNA ligand for MBP-RR-TZF (Qu et al., 2014)
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Cellular protection using Flt3 and PI3Kα inhibitors demonstrates multiple mechanisms of oxidative glutamate toxicity
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