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Then 50 μl of Ni-NTA agarose (Thermo Fisher Scientific) was added to each extract containing His-GSAAT proteins, and incubated for 2 h, as before
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CURRENT TECHNIQUES INCLUDING FINITY CHROMATOGRAPHY Currently, affinity chromatography being used for a lot of different things ranging from the study drug protein binding interactions to the depletion of high abundance proteins to enhance the detection/quantification of dilute proteins
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