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Then the cells were washed twice with HBSS, and the fluorescence images were taken using a fluorescence microscope (AXIO, Carl Zeiss Corporation, Germany)

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In the liver, rutin mitigates oxidative damage and lipid peroxidation in hepatocytes primarily through the direct scavenging of free radicals, the chelation of transition metal ions and the upregulation of endogenous antioxidant defense systems, for example superoxide dismutase (SOD) and glutathione (GSH) (73)