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The role and effectiveness of the first line defense antioxidants which basically include superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) is therefore important and indispensable in the entire defense strategy of antioxidants, especially in reference to super oxide anion radical (∗O2) which is perpetually generated in normal body metabolism through several processes

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While it was impossible to test the effect of GSSG on C127S and C127/218S mutant due to their low stability, we showed that incubation of C218S with GSSG mutant caused a decrease in activity similar to that observed for the WT ( Figure 8 )