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Detection of lipid peroxidation and reactive oxygen species by flow cytometry Lipid peroxidation in cell membranes was assessed using BODIPY 581/591 C11 (Thermo Fisher Scientific), a fluorescent probe widely used to detect ferroptosis-associated lipid peroxidation

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In experimental models, suppression of spermatogenesis in the testes and degeneration of primordial follicles and granulosa cells in the ovaries are considered hallmark features of platinum-induced gonadotoxicity.8 Chemotherapeutic agents have been shown to deplete intracellular Glutathione (GSH), one of the major endogenous antioxidants, while simultaneously increasing Myeloperoxidase (MPO) activity, an indicator of inflammatory response, and elevating Malondialdehyde (MDA), a marker of lipid peroxidation.9 Co-administration of antioxidants during chemotherapy has therefore been proposed as a strategy to mitigate oxidative stress-induced tissue injury and improve therapeutic tolerance