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4.4 Cell lysis Freshly harvested or thawed cell pellets were lysed on ice for at least 10 min with 1× quenchers (10 mM NaN 3 , 10 mM sodium (+)-l-ascorbate, 5 mM Trolox) and protease inhibitors cocktail (PIC, Roche) in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% (w/v) Triton X-100, 0.5% (w/v) sodium deoxycholate, 0.1% (w/v) SDS)

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ATF3 promotes erastin-induced ferroptosis by suppressing system Xc(.)