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3D Stirred Suspension Culture Undifferentiated LUHMES cells were inoculated as a single cell suspension spinner vessel (Corning) equipped with a magnetic paddle impeller, at a density of 1.2 × 10 5 cell mL –1 in 125 mL of proliferation medium and cultured in a humidified atmosphere of 5% CO 2 , at 37 °C
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66 Furthermore, the complex formed by uric acid and free iron ions acts as a chelating agent, effectively inhibiting the formation of free radicals from iron ions and enhancing the antioxidant effect
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