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Intestinal Models Research has investigated intestinal injury, anastomotic healing and related gastrointestinal processes

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The affinity-purified proteins were then applied to an ion exchange column (POROS 50HQ, Thermo Scientific 1255911) and eluted in 50 mM Tris-HCl pH 8.5 and 2 mM TCEP by a linear salt gradient (from 50 mM to 1000 mM NaCl)