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the first 1–12 amino acids in GPX3 and 1–54 residues in GPX6 covered the transit peptide (Lin et al., Arabidopsis GPX5 was 173 residues with probable ER or Plasma membrane localization, without transit peptide (Erfle et al., Arabidopsis GPX8 comprised of 167 amino acids with cytosolic or nuclear localization, without transit peptide (Theologis et al., S1)