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Serially diluted ligands (Acr proteins and ssDNA) were subjected to 1:1 serial dilution starting from the highest concentration in the binding buffer (20 mM Hepes pH 7.5, 150 mM KCl, 5% v/v glycerol, 2 mM DTT), yielding 16 concentration points with a gradient ranging from 0.305 nM to 10 µM
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