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The reaction outcome is determined by measuring the ratio of fluorescence emission at 445 nm (coumarin) and 520 nm (fluorescein), corresponding to the relative amounts of cleaved and intact peptides
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et al., 2021), which in turn may cause or aggravate several features possibly relevant to ME/CFS, including production of reactive oxygen species (ROS) (Corbi et al., 2013), endothelial dysfunction as well as immune dysfunction, notably involving B cells (Padro and Sanders, 2014
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